Sildenafil Inhibits Advanced Glycation End Products-induced sFlt-1 Release Through Upregulation of Heme Oxygenase-1
Journal of Menopausal Medicine
;
: 57-68, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-91564
ABSTRACT
OBJECTIVES:
We examined the effect of sildenafil citrate on advanced glycation end products (AGEs)-induced soluble fms-like tyrosine kinase 1 (sFlt-1) release in JEG-3 choriocarcinoma cells.METHODS:
Cells were incubated with control bovine serum albumin (BSA) or AGEs-BSA, and expression of sFlt-1 mRNA and protein release was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. AGEs-BSA increased sFlt-1 mRNA expression and protein release in a dose-dependent manner.RESULTS:
Sildenafil citrate suppressed sFlt-1 mRNA expression and protein release in cells treated with AGEs-BSA in a dose-dependent manner. Likewise, it inhibited the increase of reactive oxygen species (ROS) production and NF-kappaB activity in these cells. Cobalt protoporphyrin (CoPP) and bilirubin also inhibited sFlt-1 release and ROS production in cells treated with AGEs-BSA, whereas zinc protoporphyrin IX (ZnPP IX) antagonized the effect of sildenafil citrate. In cells transfected with the heme oxygenase-1 (HO-1) siRNA, sildenafil citrate failed to inhibit the sFlt-1 release and ROS production.CONCLUSION:
These results strongly suggest that sildenafil citrate inhibits sFlt-1 release and ROS production in cells treated with AGEs-BSA through upregulation of the HO-1 expression in JEG-3 cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Zinco
/
Bilirrubina
/
RNA Mensageiro
/
Soroalbumina Bovina
/
Ensaio de Imunoadsorção Enzimática
/
Coriocarcinoma
/
Regulação para Cima
/
NF-kappa B
/
Espécies Reativas de Oxigênio
/
Cobalto
Limite:
Gravidez
Idioma:
Inglês
Revista:
Journal of Menopausal Medicine
Ano de publicação:
2014
Tipo de documento:
Artigo
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