Anti-influenza Virus Activity in Agricultural, Forest and Fishery Products from Ishikawa Prefecture / 日本補完代替医療学会誌
Japanese Journal of Complementary and Alternative Medicine
; : 23-31, 2022.
Article
em Ja
| WPRIM
| ID: wpr-924390
Biblioteca responsável:
WPRO
ABSTRACT
Objective: A study was conducted to clarify the anti-influenza virus effect of agricultural, forest and fishery products in Ishikawa Prefecture. Method: The samples were prepared by both hot-water and ethanol extraction. The Madin-Darby canine kidney (MDCK) cells infected with PR8 strain were cultured in Dulbecco's Modified Eagle Medium(DMEM) containing extract for 24 hours and measured virus titer of supernatants by focus-forming reduction assay. Time-of-addition assay was performed to determine the inhibition stage in virus proliferation by the extract. The active ingredients were purified using a solid-phase extraction column (silica gel base C18). Results: We revealed that many products have anti-influenza virus activity. Especially, four products ( Matteuccia struthiopteris, Colocasia esculenta, Sagittaria trifolia, and Eleucine coracana) show strong activity and inhibit several stages in virus proliferation. Conclusion: We performed screening to 46 agricultural, forest and fishery products and found the four products ( Matteuccia struthiopteris, Colocasia esculenta, Sagittaria trifolia, and Eleusine coracana) inhibit virus proliferation strongly. In addition, the isolation and identification of the active ingredients contained in these remain as a future study.
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Índice:
WPRIM
Idioma:
Ja
Revista:
Japanese Journal of Complementary and Alternative Medicine
Ano de publicação:
2022
Tipo de documento:
Article