Genetic code expansion and its application in characterization and imaging of proteins / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 620-631, 2022.
Artigo
em Chinês
| WPRIM
| ID: wpr-927732
ABSTRACT
Genetic code expansion (GCE) allows the incorporation of unnatural amino acids into proteins via using stop codons. GCE may achieve site-specific labeling of proteins in combination with the click reaction. Compared with other labeling tools such as fluorescent proteins and tagged antibodies, the compound molecules used in protein labeling by GCE technology are smaller, and therefore, may less interfere the conformational structure of proteins. In addition, through click reaction, GCE allows a 11 stoichiometric ratio of the target protein molecule and the fluorescent dye, and the protein can be quantified based on the fluorescence intensity. Thus, GCE technology has great advantages in the researches that require the exposition of living cells under high laser power for longer time, for example, in the context of single molecule tracing and super-resolution microscopic imaging. Meanwhile, this technology lays the foundation for improving the accuracy of positioning and molecule counting in the imaging process of living cells. This review summarized the GCE technology and its recent applications in functionally characterizing, labeling and imaging of proteins.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas
/
Corantes Fluorescentes
/
Código Genético
/
Aminoácidos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2022
Tipo de documento:
Artigo
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