The signal sequence of type II porcine reproductive and respiratory syndrome virus glycoprotein 3 is sufficient for endoplasmic reticulum retention
Journal of Veterinary Science
;
: 307-313, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-92900
ABSTRACT
The glycoprotein 3 (GP3) of type II porcine reproductive and respiratory syndrome virus has the characteristic domains of a membrane protein. However, this protein has been reported to be retained in the endoplasmic reticulum (ER) rather than transported to the plasma membrane of the cell. In this study, we performed confocal laser scanning microscopy analysis of variants of GP3 and foundthat the signal sequence of the GP3 led to confinement of GP3 in the ER, while the functional ortransmembrane domain did not affect its localization. Based on these results, we concludedthat the signal sequence of GP3 contains the ER retention signal, which might play an important role in assembly of viral proteins.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Sinais Direcionadores de Proteínas
/
Linhagem Celular
/
Membrana Celular
/
Proteínas do Envelope Viral
/
Microscopia Confocal
/
Vírus da Síndrome Respiratória e Reprodutiva Suína
/
Análise de Sequência de Proteína
/
Retículo Endoplasmático
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2013
Tipo de documento:
Artigo
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