Sevoflurane regulates cell activity and apoptosis of breast cancer via Circ_001589/miR-588 / 中华内分泌外科杂志
Chinese Journal of Endocrine Surgery
;
(6): 651-656, 2021.
Artigo
em Chinês
| WPRIM
| ID: wpr-930278
ABSTRACT
Objective:
To explore the effects and molecular mechanism of circ_001598 on biological behavior of breast cancer (BC) cells.Methods:
Sevoflurane in different concentrations were used to treat BC cells and the cell activity and apoptosis were detected. qRT-PCR was used to determine the relative expression of Circ_001598 and miR-588 in BC tissue and cells. The effects of Sevoflurane on Circ_001598, miR-588 expression was detected. Dual luciferase reporter gene assay was used to detect the relationship between Circ_001598 and miR-588. The expression of Circ_001598, miR-588 in BC cells was intervened, then cell activity and apoptosis level was detected by using MTT and flow cytometry individually.Results:
Sevoflurane inhibited cell activity of BC cells, and promoted cell apoptosis. Circ_001598 was increased in BC tissue and cells, but Sevoflurane could down-regulate the expression of Circ_001598 (all P<0.05) . Overexpression of Circ_001589 partially saved the effects of Sevoflurane on cell viability and apoptosis. Circ_001598 negatively regulated miR-588 in BC cells. miR-588 expression was down-regulated in BC tissue and cells, but Sevoflurane up-regulated the expression level of miR-588 in BC cells (all P<0.05) . miR-588 transfection partially offseted the effects of Circ_001598 on Sevoflurane induced BC cells apoptosis.Conclusion:
Sevoflurane affects BC cell viability and apoptosis via regulating Circ_001589/miR-588.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Endocrine Surgery
Ano de publicação:
2021
Tipo de documento:
Artigo
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