Relationship between peripheral blood microRNA-27b expression and left ventricular hypertrophy in patients with hypertensive / 中国医师进修杂志

ABSTRACT

Objective:To investigate the correlation between peripheral blood microRNA (miR)-27b expression and left ventricular hypertrophy (LVH) in patients with hypertensive.Methods:The clinical data of 120 patients with hypertension from February 2019 to February 2021 in Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University were retrospectively analyzed. Among them, LVH occurred in 70 cases (LVH group), none in 50 cases (NLVH group). The ventricular septal thickness (VST), left ventricular posterior diastolic wall thickness (LVPWTd) and left ventricular end diastolic diameter (LVEDD) were measured by echocardiography, the left ventricular mass (LVM) and left ventricular mass index (LVMI) were calculated. The peripheral blood expression level of miR-27b was detected by real-time quantitative polymerase chain reaction. Correlation was analyzed by Pearson correlation analysis. Receiver operating characteristic (ROC) curve was drawn to evaluate the efficacy of peripheral blood miR-27b in diagnosing LVH in patients with hypertensive.Results:The peripheral blood expression level of miR-27b in LVH group was significantly higher than that in NLVH group (6.37 ± 0.23 vs. 3.42 ± 0.18), and there was statistical difference ( t = 9.58, P<0.01). Pearson correlation analysis result showed that the peripheral blood expression of miR-27b was positively correlated with LVMI, VST, LVEDD and LVPWTd in hypertensive LVH patients ( r = 0.71, 0.63, 0.75 and 0.68; P<0.01). After eliminating risk factors (duration of hypertension, systolic blood pressure, diastolic blood pressure, different medications, uric acid), multiple linear regression analysis result showed that the peripheral blood expression of miR-27b was positively correlated with LVMI, VST, LVEDD and LVPWTd in hypertensive LVH patients ( β = 0.07, 0.63, 0.42 and 0.48; P<0.01). ROC curve analysis result showed that the area under the curve of peripheral blood expression of miR-27b in predicted LVH in patients with hypertension was 0.905 (95% CI 0.854 to 0.957), the optimal cut-off value was 4.45, the sensitivity was 84.0%, and the specificity was 82.9%. Conclusions:The high peripheral blood expression of miR-27b in hypertension LVH patients is positively correlated with LVMI and other echocardiographic parameters, which has a good predictive value of LVH in patients with hypertension.