Increased p190RhoGEF expression in activated B cells correlates with the induction of the plasma cell differentiation
Experimental & Molecular Medicine
;
: 138-148, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-93416
ABSTRACT
Previously, we demonstrated that the p190 Rho guanine nucleotide exchange factor (p190RhoGEF) was induced following CD40 stimulation of B cells. In this study, we examined whether p190RhoGEF and a downstream effector molecule RhoA are required for B cell differentiation. Expression of p190RhoGEF positively correlated with the expression of surface markers and transcriptional regulators that are characteristic of mature B cells with plasma cell (PC) phenotypes. Moreover, either the overexpression of p190RhoGEF or the expression of a constitutively active RhoA drove cellular differentiation toward PC phenotypes. B cell maturation was abrogated in cells that overexpressed p190RhoGEF and a dominant-negative form of RhoA simultaneously. CD40-mediated maturation events were also abrogated in cells that overexpressed either dominant-negative p190RhoGEF or RhoA. Together, these data provide evidence that p190RhoGEF signaling through RhoA in CD40-activated B cells drives the induction of the PC differentiation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmócitos
/
Linfócitos B
/
Ativação Linfocitária
/
Diferenciação Celular
/
Linhagem Celular
/
Células Cultivadas
/
Proteína rhoA de Ligação ao GTP
/
Fatores de Troca do Nucleotídeo Guanina
/
Camundongos Endogâmicos BALB C
Limite:
Animais
/
Feminino
/
Humanos
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2012
Tipo de documento:
Artigo
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