Study on the effect and mechanism of modified Yijing decoction on the decrease of ovarian reserve function in rats / 中国药房

ABSTRACT

OBJ ECTIVE To study the effect a nd mechanism of modified Yijing decoction on the decrease of ovarian reserve function（DOR）in rats. METHODS A total of 140 SPF female SD rats were randomly divided into control group ，model group ， positive control group （Estradiol valerate tablets 0.09 mg/kg），inhibitor group [modified Yijing decoction 23.6 g/kg+transcriptional inhibitor for hypoxia inducible factor- 1α（HIF-1α）YC-1 2 mg/kg] and modified Yijing decoction low-dose ，medium-dose and high-dose groups （5.9，11.8，23.6 g/kg）. Except for control group ，rats in other groups were given cyclophosphamide via femoral vein to induce DOR model ；after successful modeling for 2 h，rats in each group were given normal saline or relevant medicine intragastrically，once a day ，for 8 consecutive weeks.The estrous cycle of rats in each group was recorded ，and the ovarian index was calculated to observe the histopathological changes and autophagy in the ovary ；serum levels of follicle stimulating hormone （FSH），luteinizing hormone （LH），anti-Müllerian hormone （AMH）and estrogen （E2）were measured ；protein and mRNA levels of HIF-1α，B lymphoblastoma 2 gene/adenovirus E 1B interacting protein 3 recombinant protein （Bnip3）and yeast Atg 6 homolog of autophagy key molecule （Beclin1）in ovarian tissues of rats were all detected. RESULTS Compared with control group ，the ovarian indexes of model group and administration groups were decreased significantly （P＜0.05）. Model group possessed disturbed estrous cycle and the ovary tissues showed pathological morphology ，the autophagolysosomes appeared in ovary cells ，AMH and E 2 levels were significantly decreased （P＜0.05）；FSH and LH levels ，protein and mRNA expressions of HIF- 1α，Bnip3 and Beclin 1 were significantly increased （P＜0.05）. Compared with model group ，the estrous cycle of rats in positive control group and modified Yijing decoction groups gradually returned to normal ，the pat hological morphology of ovarian tissue and the autophagy of ovarian cells were improved ；the ovarian index ，AMH and E 2 levels were significantly increased （P＜0.05）；the levels of FSH and LH ，protein and mRNA expressions of HIF- 1 α ， Bnip3 and Beclin 1（except for protein of HIF- 1α，protein and wanyunhui188@163.com mRNA of Bnip 3 in modified Yijing decoction low-dose group ） were decreased significantly （P＜0.05）. The protein expressions of H IF-1α，Bnip3 and Beclin 1，mRNA expressions of HIF- 1α and Beclin 1 were decreased significantly in the inhibitor group （P＜0.05）. CONCLUSIONS Modified Yijing decoction can improve ovarian function ，regulate the secretion of sex hormone ，and protect ovarian tissue in DOR model rats ，and the mechanism of which may be related to the inhibition of HIF- 1α/ Bnip3/Beclin1 pathway.