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Pathogenic detection and molecular tracing of a bacillary dysentery outbreak by Shigella sonnei in Huainan city / 中华预防医学杂志
Chinese Journal of Preventive Medicine ; (12): 437-442, 2022.
Artigo em Chinês | WPRIM | ID: wpr-935304
ABSTRACT

Objective:

To understand the virulence gene and drug resistance profile of Shigella sonnei outbreak in Huainan city, and conduct pathogenic traceability analysis.

Methods:

Water samples and feces related to an infectious diarrhea outbreak in Huainan city in August 2020 were collected for multiple pathogen detection. Virulence gene, drug sensitivity, pulse-field gel electrophoresis and whole genome sequencing of Shigella isolates were analyzed respectively.

Results:

38 strains of Shigella sonnei were detected in 56 samples of mucilage feces with a positive rate 67.86%, and all serotypes were Shigella sonnei Phase I. Three strains of Shigella sonnei were detected by fluorescence PCR in the Gram-negative (GN) bacterial enrichment solution of terminal water and well water. Virulence genes were ipaH positive (38), ipaH/ial (31) and ipaH/ial/sen positive (1), respectively. The drug resistance spectrum showed that 9 of 14 antibiotics were 100% resistant, and only imipenem, chloramphenicol, ceftazidime and ciprofloxacin were effective drugs. XbaⅠ restriction enzyme map type of 36 isolates was completely consistent, and the ST type analysis of 3 strains was ST152. Whole genome sequencing and analysis verified that the outbreak was caused by a single clonal group of strains, and revealed that the isolates of the outbreak were clustered into a large cluster with 3 Chinese strains and 1 Korean strain in the database, far away from the strains of other countries.

Conclusion:

The outbreak is caused by a single clone of Shigella sonnei, which are low virulence strains and have multiple drug resistance.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Shigella / Shigella sonnei / Água / Surtos de Doenças / Disenteria Bacilar Tipo de estudo: Estudo diagnóstico Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Preventive Medicine Ano de publicação: 2022 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Shigella / Shigella sonnei / Água / Surtos de Doenças / Disenteria Bacilar Tipo de estudo: Estudo diagnóstico Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Preventive Medicine Ano de publicação: 2022 Tipo de documento: Artigo