Capsaicin-Induced Apoptosis of FaDu Human Pharyngeal Squamous Carcinoma Cells
Yonsei Medical Journal
;
: 834-841, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-93570
ABSTRACT
PURPOSE:
To investigate the anti-tumor effect of capsaicin on human pharyngeal squamous carcinoma cells (FaDu). MATERIALS ANDMETHODS:
The expression of apoptosis/cell cycle-related proteins (or genes) was examined by reverse transcriptase-polymerase chain reaction, western blotting and ELISA methods, while the apoptotic cell population, cell morphology and DNA fragmentation levels were assessed using flow cytometry, fluorescence microscopy and agarose gel electrophoresis.RESULTS:
Capsaicin was found to inhibit the growth and proliferation of FaDu cells in a dose- and time-dependent manner. Apoptotic cell death was confirmed by observing increases in nuclear condensation, nuclear DNA fragmentation and sub-G1 DNA content. The observed increase in cytosolic cytochrome c, activation of caspase 3 and PARP (p85) levels following capsaicin treatment indicated that the apoptotic response was mitochondrial pathway-dependent. Gene/protein expression analysis of Bcl-2, Bad and Bax further revealed decreased anti-apoptotic Bcl-2 protein and increased pro-apoptotic Bad/Bax expression. Furthermore, capsaicin suppressed the cell cycle progression at the G1/S phase in FaDu cells by decreasing the expression of the regulators of cyclin B1 and D1, as well as cyclin-dependent protein kinases cdk-1, cdk-2 and cdk-4.CONCLUSION:
Our current data show that capsaicin induces apoptosis in FaDu cells and this response is associated with mitochondrial pathways, possibly by mediating cell cycle arrest at G1/S.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Capsaicina
/
Carcinoma de Células Escamosas
/
Neoplasias Faríngeas
/
Ciclo Celular
/
Western Blotting
/
Apoptose
/
Proteínas Proto-Oncogênicas c-bcl-2
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Linhagem Celular Tumoral
Limite:
Humanos
Idioma:
Inglês
Revista:
Yonsei Medical Journal
Ano de publicação:
2012
Tipo de documento:
Artigo
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