Your browser doesn't support javascript.
loading
A study on osteoblast-like cell responses to surface-modified titanium / 대한치과보철학회지
The Journal of Korean Academy of Prosthodontics ; : 300-318, 2003.
Artigo em Inglês | WPRIM | ID: wpr-93691
ABSTRACT
STATEMENT OF

PROBLEM:

The success of implants depends on intimate and direct contact of implant material on bone tissue and on functional relationship with soft tissue contact. Creation and maintenance of osseointegration depend on the understanding of the tissue's healing, repairing, and remodeling capacity and these capacities rely on cellular behavior. Altering the surface properties can modify cellular responses such as cell adhesion, cell motility, bone deposition. Therefore, various implant surface treatment methods are being developed for the improved bone cell responses.

PURPOSE:

The purpose of this study was to evaluate the responses of osteoblast-like cells to surface- modified titanium. MATERIALS AND

METHODS:

The experiment was composed of four groups. Group 1 represented the electropolished surface. Group 2 surfaces were machined surface. Group 3 and Group 4 were anodized surfaces. Group 3 had low roughness and Group 4 had high roughness. Physicochemical properties and microstructures of the d iscs were examined and the responses of osteoblast-like cells to the discs were investigated. The microtopography was observed by SEM. The roughness was measured by three-dimension roughness measuring system. The microstructure was analyzed by XRD, AES. To evaluate cell responses to modified titanium surfaces, osteoblasts isolated from calvaria of neonatal rat were cultured. Cell count, morphology, total protein measurement and alkaline phosphatase activities of the cultures were examined. RESULTS AND

CONCLUSION:

The results were as follows 1. The four groups showed specific microtopography respectively. Anodized group showed grain structure with micropores. 2. Surface roughness values were, from the lowest to the highest, electropolished group, machined group, low roughness anodized group, and high roughness anodized group. 3. Highly roughened anodized group was found to have increased surface oxide thickness and surface crystallinity. 4. The morphology of cells, flattened or spherical, were different from ach other. In the electropolished group and machined group, the cells were almost flattened. In two anodized groups, some cells were spherical and other cells were flattened. And the 14 day culture cells of all of the groups were nearly flattened due to confluency. 5. The number of attached cells was highest in low roughness anodized group. And the machined group had significantly lower cell count than any other groups(P<.05). 6. Total protein contents showed no difference among groups. 7. The level of alkaline phosphatase activities was higher in the anodized groups than electropolished and machined groups(P<.05).
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Osteoblastos / Crânio / Propriedades de Superfície / Titânio / Osso e Ossos / Adesão Celular / Contagem de Células / Movimento Celular / Grão Comestível / Osseointegração Limite: Animais Idioma: Inglês Revista: The Journal of Korean Academy of Prosthodontics Ano de publicação: 2003 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Osteoblastos / Crânio / Propriedades de Superfície / Titânio / Osso e Ossos / Adesão Celular / Contagem de Células / Movimento Celular / Grão Comestível / Osseointegração Limite: Animais Idioma: Inglês Revista: The Journal of Korean Academy of Prosthodontics Ano de publicação: 2003 Tipo de documento: Artigo