The Effects of Amniotic Membrane Contact Lens for Cornea Wound Healing
Journal of the Korean Ophthalmological Society
; : 989-995, 2009.
Article
em Ko
| WPRIM
| ID: wpr-94270
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: To investigate the efficacy of an amniotic membrane contact lens on corneal epithelial wound healing. METHODS: We made a model with a corneal epithelial wound by applying 6 mm round filter paper soaked with 1 N NaOHonto the central cornea in 24 eyes of 12 rabbits. The rabbits were divided into three groups: AMCL (amniotic membrane contact lens), T-AMT (temporary amniotic membrane transplantation) and the control group. We evaluated corneal wound healing every postoperative day using a digital photo slitlamp and fluorescein dye. The corneas were harvested for histopathologic studies after seven days and analyzed with hematoxylin-eosin (H & E) stain and TUNEL staining. RESULTS: The average wound healing time was similar between the amniotic membrane contact lens and the temporary amniotic membrane transplantation group. The number of the infiltrated PMNs (polymorphonuclear cells) was 8.8+/-2.58, 8.6+/-2.19 and 48.6+/-7.12 in the AMCL, T-AMT and control groups, respectively. Apoptotic keratocytes were 3.8+/-1.1, 3.6+/-1.09 and 23.2+/-5.06 in the AMCL, T-AMT and control groups, respectively. In the AMCL and T-AMT groups, the number of infiltrated PMNs and apoptotic keratocytes were significantly less than those the control group (p<0.05). There were not significant differences in the number of PMNs and apoptotic cells in the AMCL and the T-AMT groups. CONCLUSIONS: Amniotic membrane contact lenses have the benefits of being an easily applied method and having a wound healing ability comparable to that possible with conventional suture methods.
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
Suturas
/
Cicatrização
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Lentes de Contato
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Córnea
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Fluoresceína
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Transplantes
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Marcação In Situ das Extremidades Cortadas
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Olho
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Âmnio
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Membranas
Limite:
Animals
Idioma:
Ko
Revista:
Journal of the Korean Ophthalmological Society
Ano de publicação:
2009
Tipo de documento:
Article