Application of fingerprint combined with quantitative analysis of multi -components by single marker in quality evaluation of Xanthoceras sorbifolia / 中国药房

ABSTRACT

OBJECTIVE To establish the fingerprints of Xanthoceras sorbifolia and determine the contents of flavonoids . METHODS HPLC was adopted . Using epigallocatechin as reference ，the fingerprints of 11 batches（No. S1-S11）of X. sorbifolia were drawn with Similarity Evaluation System of Chromatographic Fingerprints of TCM （2004A edition ）. The similarity evaluation was conducted ，the common peaks were also confirmed . Cluster analysis （CA）and principal component analysis （PCA）were also performed. Epigallocatechin was selected as internal reference ，and quantitative analysis of multi -components by single marker （QAMS）was used to determine the contents of gallocatechin ，catechin，epicatechin，dihydromyricetin，taxifolin and myricetin in 16 batches（No. S1-S16）of X. sorbifolia. The results were compared with the results of one point external standard method and standard curve method . RESULTS There were 15 common peaks in 11 batches of X. sorbifolia，and the similarity of them were 0.910-1.000. A total of 7 common peaks were identified ，i.e. galliccatechin（peak 1），epigallocatechin（peak 2），catechin（peak 3），epicatechin（peak 5），dihydromyricetin（peak 6），taxifolin（peak 14）and myricetin （peak 15）. The results of CA showed that S5-S7 and S 9 were clustered into one category ，S8 and S 11 were clustered into one category ，S10 were clustered into one category，S1-S4 were clustered into one category . The results of PCA showed that accumulative variance contribution rate of 3 principal components was 99.24%；S5-S7 were clustered into one category ，S8-S11 were clustered into one category ，S3 and S 4 were clustered into one category ，S1 and S 2 were clustered into one category . With the exception of myricetin and a partial batches （S12，S14-S16） of catechin ，the RSDs measured by the three methods for galliccatechin ，catechin （remaining batches ）， epicatechin，dihydromyricetin and taxifolin in 16 batches of X. sorbifolia were less than 4% （n＝3）. CONCLUSIONS The established HPLC fingerprint and the method for content determination can be used for the quality control of X. sorbifolia. QAMS method can be used for the content determination of galliccatechin，epicatechin，dihydromyricetin and taxifolin .