Effects of Polyamines on TNFalpha- or Tamoxifen-induced Apoptosis in Human Breast Cancer Cells / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment
;
: 385-391, 2001.
Artigo
em Coreano
| WPRIM
| ID: wpr-94698
ABSTRACT
PURPOSE:
To investigate the effects of polyamines on tumor necrosis factor alpha (TNFalpha)-or tamoxifen (TAM)-induced apoptosis in estrogen receptor (ER)-positive MCF- 7 and ER-negative MDA-MB-231 human breast cancer cells. MATERIALS ANDMETHODS:
Cell viability was assessed by using MTT assay. Reactive oxygen species (ROS) generation was measured using 2', 7'-dichlorofluorescin diacetste (DCFDA) by fluorescence plate reader. DNA fragmentation was assessed by 1.5% agarose gel electrophoresis.RESULTS:
TNFalpah and TAM showed significant dose- and time- dependent inhibitory effects on the growth of MCF-7 human cells. However, the growth of MDA-MB-231 cells were not inhibited by TNFalpha or TAM treatment. The generation of ROS was increased in dose-and time-dependent manner by TNFalpha treatment in MCF-7 cells. Polyamines, especially spermine suppressed TNFalpha-induced ROS generation in MCF-7 cells. Antioxidant effects of polyamines were also demonstrated by DNA fragmentation, cell morphology as well as ROS generation assay. Polyamines also blocked TAM-induced cell death in MCF-7 cell. However, MDA-MB-231 cells showed resistance to the cytotoxic effects of TNFalpha or TAM.CONCLUSION:
These results suggest that polyamines may prevent TNFalpha or TAM-induced apoptosis in MCF-7 human breast cancer cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Poliaminas
/
Tamoxifeno
/
Mama
/
Neoplasias da Mama
/
Espermina
/
Sobrevivência Celular
/
Fator de Necrose Tumoral alfa
/
Morte Celular
/
Espécies Reativas de Oxigênio
/
Apoptose
Limite:
Humanos
Idioma:
Coreano
Revista:
Cancer Research and Treatment
Ano de publicação:
2001
Tipo de documento:
Artigo
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