Effects of hepatitis C virus NS3/4A protein on proliferation, apoptosis and division of human hepatocarcinoma SMMC-7721 cells / 肿瘤研究与临床

ABSTRACT

Objective:To investigate the effects of hepatitis C virus (HCV) NS3/4A protein on proliferation, apoptosis and division of human hepatocarcinoma SMMC-7721 cells.Methods:The recombinant plasmid pLV-Green-NS3/4A and pLV-puro-NS3/4A were produced by homologous recombination method, and then the cells were divided into pLV-Green-NS3/4A or pLV-puro-NS3/4A transfection group and their corresponding control group (transfected with empty vector). The expression of recombinant protein was detected by immunofluorescence staining and Western blotting. Methyl thiazol tetrazolium (MTT) method was used to detect cell proliferation, DAPI staining was used to detect cell apoptosis, and immunofluorescence was used to analyze cell devision.Results:The growth state of SMMC-7721 cells in the pLV-Green-NS3/4A transfection group was worse than that in the corresponding empty vector control group. Compared with the corresponding empty vector control group, the proliferation activity of SMMC-7721 cells in the pLV-Green-NS3/4A transfection group on the 3rd and 4th day was inhibited (both P < 0.05). The apoptosis rates of cells in the pLV-Green-NS3/4A transfection group and the corresponding empty vector control group were (20.3±3.5)% and (5.3±1.5)%, and the difference was statistically significant ( t = -8.57, P < 0.001). Compared with the corresponding empty vector control group, the cells in the pLV-puro-NS3/4A transfection group had more multipolar spindles, and the multipolar division rates of the two groups were (2.33±0.58)% and (6.01±0.99)%, and the difference was statistically significant ( t = -5.50, P = 0.005). Conclusions:HCV NS3/4A can inhibit the proliferation, promote the apoptosis and multipolar division of human hepatocarcinoma SMMC-7721 cells.