Identification of differentially expressed cDNAs in Acanthamoeba culbertsoni after mouse brain passage
The Korean Journal of Parasitology
;
: 15-20, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-96038
ABSTRACT
Free-living amoebae of the genus Acanthamoeba are causative agents of granulomatous amebic encephalitis and amebic keratitis. Because the virulence of Acanthamoeba culbertsoni cultured in the laboratory is restored by consecutive brain passages, we examined the genes induced in mouse brain-passaged A. culbertsoni by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR). Enhanced A. culbertsoni virulence was observed during the second mouse brain passage, i.e., infected mouse mortality increased from 5% to 70%. Ten cDNAs induced during mouse brain passage were identified by DDRT-PCR and this was confirmed by northern blot analysis. BlastX searches of these cDNAs indicated the upregulations of genes encoding predictive NADH-dehydrogenase, proteasomal ATPase, and GDP-mannose pyrophosphorylase B, which have previously been reported to be associated with A. culbertsoni virulence factors.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Virulência
/
Encéfalo
/
Acanthamoeba
/
Dados de Sequência Molecular
/
Inoculações Seriadas
/
Regulação para Cima
/
Regulação da Expressão Gênica
/
Northern Blotting
/
DNA de Protozoário
/
Clonagem Molecular
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2006
Tipo de documento:
Artigo
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