Serum protein-binding of radioactive triiodothyronine in the evaluation of thyroid function using sephadex gel filtration

ABSTRACT

1. A new technique for the direct measurement of T3-serum protein complex has been devised and perfected. This procedure has several distinct advantages over the standard procedures (a) previous iodide intake (inorganic or organic)does not vitiate the result, (b) no radioactivity is introduced into the system of the patient, (c) many variables connected with red cell or resin uptake procedures are obviated, and (d) a shorter period of time is required, 3 hours, to finish the test2. A total of 160 test runs were made on 136 subjects using this new procedure. Results revealed highly significant values for the evaluation of thyroid function3. In certain situations where probably either alterations in TBP thyroxine binding capacity or changes in the proportion of T4 and T3 are operative, T8-binding gives reliable results, where PBI does not .(Summary)