Heterogeneous Composition of Voltage-Dependent K+ Currents in Hepatic Stellate Cells
Yonsei Medical Journal
; : 684-693, 2007.
Article
em En
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| ID: wpr-96526
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WPRO
ABSTRACT
PURPOSE: Hepatic stellate cells (HSC) are a type of pericyte with varying characteristics according to their location. However, the electrophysiological properties of HSC are not completely understood. Therefore, this study investigated the difference in the voltage-dependent K(+) currents in HSC. MATERIALS AND METHODS: The voltage-dependent K(+) currents in rat HSC were evaluated using the whole cell configuration of the patch-clamp technique. RESULTS: Four different types of voltage-dependent K(+) currents in HSC were identified based on the outward and inward K(+) currents. Type D had the dominant delayed rectifier K(+) current, and type A had the dominant transient outward K(+) current. Type I had an inwardly rectifying K(+) current, whereas the non-type I did not. TEA (5mM) and 4-AP (2mM) suppressed the outward K(+) currents differentially in type D and A. Changing the holding potential from -80 to -40mV reduced the amplitude of the transient outward K(+) currents in type A. The inwardly rectifying K(+) currents either declined markedly or were sustained in type I during the hyperpolarizing step pulses from -120 to -150mV. CONCLUSION: There are four different configurations of voltage-dependent K(+) currents expressed in cultured HSC. These results are expected to provide information that will help determine the properties of the K(+) currents in HSC as well as the different type HSC populations.
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WPRIM
Assunto principal:
Células Cultivadas
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Transporte de Íons
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Técnicas de Patch-Clamp
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Hepatócitos
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Canais de Potássio de Abertura Dependente da Tensão da Membrana
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Condutividade Elétrica
Limite:
Animals
Idioma:
En
Revista:
Yonsei Medical Journal
Ano de publicação:
2007
Tipo de documento:
Article