Delayed allograft rejection by the suppression of class II transactivator
Experimental & Molecular Medicine
;
: 210-216, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-96570
ABSTRACT
We examined the effect of class II transactivator (CIITA) down-modulation on allograft rejection. To inhibit the function of CIITA, we constructed a series of CIITA mutants and found one exhibiting the dominant-negative effect on the regulation of major histocompatibility complex (MHC) class II expression. To test whether the CIITA dominant-negative mutant reduces immunogenecity, CIITA-transfected melanoma cells were injected into allogeneic host and assessed for immune evading activity against host immune cells. We demonstrated that the CIITA dominant-negative mutant allowed tumor nodules to develop earlier in the lung than control by this tumor challenge study. Furthermore, skin grafts deficient for CIITA also survived longer than wild-type in allogeneic hosts. Both the tumor challenge and skin graft studies suggest the inhibition of CIITA molecules in donor tissue would be beneficial to the control of allo-response.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transplante Homólogo
/
Melanoma Experimental
/
Camundongos Transgênicos
/
Proteínas Nucleares
/
Transfecção
/
Antígenos de Histocompatibilidade Classe II
/
Ativação Transcricional
/
Transativadores
/
Interferon gama
/
Transplante de Pele
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2006
Tipo de documento:
Artigo
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