Problems Associated with I-125 Oxytocin Binding to Membrane Receptors
Yonsei Medical Journal
;
: 24-35, 1980.
Artigo
em Inglês
| WPRIM
| ID: wpr-96980
ABSTRACT
Radioiodinated oxytocin prepared by the lactoperoxidase method exhibited a substantial biologic activity in uterotonic assay of the rat uterus. 125I-oxytocin was bound to the uterine membrane particulate fraction, but the unlabelled oxytocin did not inhibit the binding of 125I oxytocin to the membrane fraction of rat uterus. Cold iodinated oxytocin, however, inhibited the 125I-oxytocin binding to the membrane fraction of rat uterus in proportion to its concentration. These results suggest that 125I-oxytocin is not a suitable radioligand for oxytocin receptor binding study.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ensaio Radioligante
/
Útero
/
Sítios de Ligação
/
Ocitocina
/
Membrana Celular
/
Receptores de Superfície Celular
/
Radioisótopos do Iodo
/
Animais
Limite:
Animais
Idioma:
Inglês
Revista:
Yonsei Medical Journal
Ano de publicação:
1980
Tipo de documento:
Artigo
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