Advances in heterologous expression, structural elucidation and molecular modification of pullulanase / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 4432-4448, 2022.
Artigo
em Chinês
| WPRIM
| ID: wpr-970325
ABSTRACT
Starch is composed of glucose units linked by α-1, 4-glucoside bond and α-1, 6-glucoside bond. It is the main component of foods and the primary raw material for starch processing industry. Pullulanase can effectively hydrolyze the α-1, 6-glucoside bond in starch molecules. Combined with other starch processing enzymes, it can effectively improve the starch utilization rate. Therefore, it has been widely used in the starch processing industry. This paper summarized the screening of pullulanase-producing strain and its encoding genes. In addition, the effects of expression elements and fermentation conditions on the production of pullulanase were summarized. Moreover, the progress in crystal structure elucidation and molecular modification of pullulanase was discussed. Lastly, future perspectives on pullulanase research were proposed.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Amido
/
Glicosídeo Hidrolases
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2022
Tipo de documento:
Artigo
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