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Effect of pulsed electromagnetic fields on mesenchymal stem cell-derived exosomes in inhibiting chondrocyte apoptosis / 生物医学工程学杂志
Journal of Biomedical Engineering ; (6): 95-102, 2023.
Artigo em Chinês | WPRIM | ID: wpr-970678
ABSTRACT
The study aims to explore the effect of mesenchymal stem cells-derived exosomes (MSCs-Exo) on staurosporine (STS)-induced chondrocyte apoptosis before and after exposure to pulsed electromagnetic field (PEMF) at different frequencies. The AMSCs were extracted from the epididymal fat of healthy rats before and after exposure to the PEMF at 1 mT amplitude and a frequency of 15, 45, and 75 Hz, respectively, in an incubator. MSCs-Exo was extracted and identified. Exosomes were labeled with DiO fluorescent dye, and then co-cultured with STS-induced chondrocytes for 24 h. Cellular uptake of MSC-Exo, apoptosis, and the protein and mRNA expression of aggrecan, caspase-3 and collagenⅡA in chondrocytes were observed. The study demonstrated that the exposure of 75 Hz PEMF was superior to 15 and 45 Hz PEMF in enhancing the effect of exosomes in alleviating chondrocyte apoptosis and promoting cell matrix synthesis. This study lays a foundation for the regulatory mechanism of PEMF stimulation on MSCs-Exo in inhibiting chondrocyte apoptosis, and opens up a new direction for the prevention and treatment of osteoarthritis.
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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Apoptose / Condrócitos / Campos Eletromagnéticos / Exossomos / Células-Tronco Mesenquimais Limite: Animais Idioma: Chinês Revista: Journal of Biomedical Engineering Ano de publicação: 2023 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Apoptose / Condrócitos / Campos Eletromagnéticos / Exossomos / Células-Tronco Mesenquimais Limite: Animais Idioma: Chinês Revista: Journal of Biomedical Engineering Ano de publicação: 2023 Tipo de documento: Artigo