Identification and Bioinformatics Analysis of CYP71 Gene Family of Diploid Perilla frutescens / 中国实验方剂学杂志

ABSTRACT

ObjectiveCYP71 gene family is one of the CYP71 clans belonging to cytochrome P450， which plays an important role in secondary metabolites， especially terpenoid biosynthesis. To understand the characteristics of CYP71 family of diploid Perilla frutescens and predict its function， this study identified and systematically analyzed the family by bioinformatics. MethodOn the basis of the whole genome of diploid P. frutescens PC99， the conserved domains of CYP71 family of diploid P. frutescens were screened， and the sequence characteristics， gene structure， chromosome location， phylogeny and cis-acting elements were analyzed by National Center for Biotechnology Information （NCBI）， TBtools， MEME， Molecular Evolutionary Genetics Analysis （MEGA）， Cytoscape and other tools. ResultA total of 68 CYP71 genes were identified from diploid P. frutescens， which were unevenly distributed on 34 chromosomes and belonged to two subfamilies. They encoded 481-530 amino acids and contained 10 conserved motifs， with the isoelectric point of 5.70-9.03 and the molecular weight of 54 217.07-60 031.79 Da. The enrichment analysis and functional annotation analysis revealed 11 enriched pathways and 114 categories， and the genes were mainly annotated in biological processes. There were many cis-acting elements in the promoter region of CYP71， mainly light-responsive and methyl jasmonate-responsive elements. Protein-protein interaction analysis indicated that CYP71 protein had multiple functions such as terpene cyclase activity. ConclusionThis study lays a foundation for the functional study of CYP71 family， and provides a reference for the biosynthesis of monoterpenes in P. frutescens and the directional cultivation of excellent varieties.