Chloride channel involved in the regulation of curcumin-induced apoptosis of human breast cancer cells
Asian Pacific Journal of Tropical Medicine
; (12): 240-244, 2018.
Article
em Zh
| WPRIM
| ID: wpr-972476
Biblioteca responsável:
WPRO
ABSTRACT
Objective: To investigate the role of ClC-3 chloride channel in the proliferation of breast cancer cell line Mcf-7 treated with curcumin and its specific mechanism. Methods: MTT assay was used to detect the effect of chloride channel blocker (DIDS) and curcumin on Mcf-7 and human normal cell viability. Patch-clamp technique was used to determine the current density before and after drug treatment. Apoptosis assay by flow cytometry was performed for further examination of cell apoptosis. Results: Curcumin had toxicity on Mcf-7 and HUVEC cells and DIDS reduced the survival rate of Mcf-7 cells by inhibiting proliferation. Curcumin could activate the chloride ion current on MCF-7 cell membrane, which would be inhibited by DIDS. Finally, curcumin in low concentration combined with DIDS could significantly promote the MCF-7 cells apoptosis. Conclusions: Our results suggest that ClC-3 protein is involved in the regulation of curcumin induced proliferation inhibiting in breast cancer cells through inducing cell apoptosis. ClC-3 may be a potential target of tumor therapy.
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1
Índice:
WPRIM
Idioma:
Zh
Revista:
Asian Pacific Journal of Tropical Medicine
Ano de publicação:
2018
Tipo de documento:
Article