Effect of Immunos on immune response cells by azathioprine Induced immunosuppression in mice / Монголын Анагаах Ухаан

ABSTRACT

Background@#Herbal medicines continue to be widely used as natural promoters of good health, as immune-modulators in recent years. This situation is directly related to the rapid growth of natural based products, the decrease of chemical synthesized products and as well as the increase of natural substance consumption. @*Objective@#The purpose of this survey was to study influence of Immunos herbal medicines on immune system in the experimental and preclinical circumstances.@*Materials and Methods@#The immune deficiency was to created by Azathioprine through 5 days in the white mice after that control group, preparation of Immunal, Salimon and Immunos 1, 2 were administrated appropriate doses by oral during 10 days. Then we collected blood and quantified number of white blood cells (K/µL), quantity of splenocyte (×106 cell/ml), amount of CD4+, CD8+ and IgM, IgA, Ig G (mg/ml) (Elisa Kit Assay Catalog. No WAM-568 (Elisa Reader, 450 nm)-WKEA MED SUPPLIES CORP) on the 5th, 10th days.@*Results@#All statistical analyses were conducted with SPSS version 20.0 software (IBM, Armonk, NY). One-way ANOVA was used to assess statistical significance between Immunos groups and days of observation. Mean values of white blood cells in blood, quantity of splenocyte, CD4+, CD8+ and IgM, IgG levels determined in the control and experimental groups. White blood cells level were significantly increased in the Immunos group compared with the control group by 55.6 percent (11.5±0.9 K/µL vs 5.1±0.51 K/µL, p<0.001) and number of splenocyte increased Immunos group compared with the control group by 60.6 % (352.2±23.5 ×106 cell/ml vs 138.6±23.5 ×106 cell/ml, p<0.01). Therefore, CD4+, CD8+ and IgM, IgG levels were significantly increased in the Immunos group compared with the control group by 0.71 to 8.8% (IgG 11.47±0.42 vs 10.45±0.43 μg/ml, IgM 11.33±0.81 vs 10.48±0.31 μg/ml, CD4+ 10.44±0635 vs 10.04±0.372 U/ml, CD8+ 9.75±1.02 vs 9.68±0.45 U/ml p<0.02).@*Conclusion@#It’s concluded that, Immunos preparation shows immune-stimulator effect in cellular immunity and humoral immunity in the case of immunosuppressant by Azathioprine.