Influence of Morroniside on the Growth and Protection Against H_2O_2-Induced Cytotoxicity in SH-SY5Y Nerve Cells / 中国康复理论与实践

ABSTRACT

@#Objective To investigate the effect of Morroniside which was isolated from Cornus officinalis on the growth and the protection against H2O2-induced injury in SH-SY5Y neuroblastoma cells.Methods SH-SY5Y cells were incubated with Morroniside in different concentration.MTT metabolic rate was measured as the cell survival rate in order to determine the best effective concentration to promote the survival of neuron.MTT metabolic rate,axonal length,area of cell body and cell counting were used as the indicators to analyze the effects of Morroniside on the growth of SH-SY5Y on the best effective concentration.After SH-SY5Y cells were injured with 500 μmol/L H2O2,they were administrated with Morroniside in different concentration and vitamin E.The expression of neural growth factor(NGF)were determined with Western Blotting.Results Morroniside increased the cell survival rate in 10～100 μmol/L.Compared with control group,MTT metabolic rate(P<0.05),axonal length(P<0.001),area of cell body(P<0.01),cell counting(P<0.05)increased in morroniside-treated group after SH-SY5Y cells were incubated with Morroniside in 10 μmol/L.The expression of NGF in morroniside-treated group was higher than that of the control group after injury induced by H2O2.Conclusion Morroniside shows the neurotrophic effect and can protect SH-SY5Y cells from injury induced by H2O2.