Characteristic of growth decreased CHO(dhfr-) cell line mutated by outer space / 中国康复理论与实践
Chinese Journal of Rehabilitation Theory and Practice
;
(12): 647-648, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-979515
ABSTRACT
@#ObjectiveTo investigate the changes of the morphology, growth and cycle of CHO(dhfr-) cells after space flight.MethodsCHO(dhfr-) cells were carried in the No.18 recoverable satellite and monocloned harvesting cells before multiplying. 4 cell lines were selected randomly,and the growth characteristics of the most slowly growing one at the fifth passage was observed by methods of MTT and FCM as well as the cells' shape.Results159 cell strains were obtained after monocloning and multiplying. The cells' morphology changes, growth speed decrease and the number of G1 phase increased markedly.ConclusionSpace flight induced morphological changes of cells and it is impossible to screen out finer bioengineering cells.
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Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Rehabilitation Theory and Practice
Ano de publicação:
2004
Tipo de documento:
Artigo
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