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Application of virus-induced gene silencing technology to investigate the phytochrome metabolism mechanism: a review / 生物工程学报
Chinese Journal of Biotechnology ; (12): 2579-2599, 2023.
Artigo em Chinês | WPRIM | ID: wpr-981217
ABSTRACT
Color is an important indicator for evaluating the ornamental traits of horticultural plants, and plant pigments is a key factor affecting the color phenotype of plants. Plant pigments and their metabolites play important roles in color formation of ornamental organs, regulation of plant growth and development, and response to adversity stress. It has therefore became a hot topic in the field of plant research. Virus-induced gene silencing (VIGS) is a vital genomics tool that specifically reduces host endogenous gene expression utilizing plant homology-dependent defense mechanisms. In addition, VIGS enables characterization of gene function by rapidly inducing the gene-silencing phenotypes in plants. It provides an efficient and feasible alternative for verifying gene function in plant species lacking genetic transformation systems. This paper reviews the current status of the application of VIGS technology in the biosynthesis, degradation and regulatory mechanisms of plant pigments. Moreover, this review discusses the potential and future prospects of VIGS technology in exploring the regulatory mechanisms of plant pigments, with the aim to further our understandings of the metabolic processes and regulatory mechanisms of different plant pigments as well as improving plant color traits.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Vírus de Plantas / Plantas / Regulação da Expressão Gênica de Plantas / Inativação Gênica / Desenvolvimento Vegetal / Vetores Genéticos Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2023 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Vírus de Plantas / Plantas / Regulação da Expressão Gênica de Plantas / Inativação Gênica / Desenvolvimento Vegetal / Vetores Genéticos Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2023 Tipo de documento: Artigo