Expression of EIIIA-fibronectin in injured rat skin used in estimation of wound interval / 法医学杂志

ABSTRACT

OBJECTIVE@#To observe the means of fibronectin(FN) alternative splicing and the expression of EIIIA-FN variant in rat skin after bruise, for the sake of providing some help for forensic estimation of wound interval.@*METHODS@#Total RNA was isolated from wounded skin, and reverse transcription polymerase chain reaction was conducted to amplify target segments.@*RESULTS@#Detectable EIIIA+(526 bp) segments, lacked in normal organize, was amplified at 1 h after experimental wound, and the levels were increased within 24 h.@*CONCLUSION@#The alternative splicing EIIIA-fibronectin variant would be a satisfied criterion for research of skin injury.