New improved approaches for DYS385 detection / 法医学杂志
Journal of Forensic Medicine
;
(6): 27-29, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-982965
ABSTRACT
OBJECTIVE@#To establish more sensitive methods for detection of DYS385 in routine forensic casework.@*METHODS@#The primers recommended by Genome Database (GDB) and Schneider were used to amplify DYS385 respectively. Then, a semi-nested PCR of DYS385 was designed by using the two different primers as outer and inner primer. A series of experiments were carried out to achieve good result by adjusting the ratio of outer/inner primer and optimizing the PCR condition.@*RESULTS@#It showed that an overall 112 bp shorter DYS385 fragments and better electrophoretic separation were obtained by using primer2B. By using the semi-nested PCR approach, the shorter specific DYS385 fragments could be amplified and detectable DNA amounted to 50 pg.@*CONCLUSION@#This method is 20 fold more sensitive than the ordinary method.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Reação em Cadeia da Polimerase
/
Análise de Sequência de DNA
/
Sequências de Repetição em Tandem
/
Cromossomos Humanos Y
/
Medicina Legal
/
Frequência do Gene
Limite:
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2003
Tipo de documento:
Artigo
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