Genotype reliability of short tandem repeats typing from minute DNA / 法医学杂志
Journal of Forensic Medicine
;
(6): 151-153, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-982990
ABSTRACT
OBJECTIVE@#To understand the forensic practice of DNA profiling from minimal amount of DNA.@*METHODS@#Serial dilutions of DNA were amplified with the PowerPlex 16 System Kit, then the genotyping of short tandem repeat(STR) was performed by ABI 377 DNA automated Sequencer.@*RESULTS@#When the mount of DNA template was less than 250 pg, allelic drop-out apparently occurred at several loci. Other disturbed peaks, such as artefact bands and imbalanced heterozygote, also presented.@*CONCLUSION@#The anomalous results may result in incorrect genotyping. Careful and comprehensive considerations are needed to interpret the STR profile of minute DNA.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
DNA
/
Análise de Sequência de DNA
/
Repetições Minissatélites
/
Sequências de Repetição em Tandem
/
Alelos
/
Medicina Legal
/
Genótipo
Limite:
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2003
Tipo de documento:
Artigo
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