The expression of Bcl-2 protein on acute myocardial ischemia/reperfusion and the significance in forensic pathology / 法医学杂志

ABSTRACT

OBJECTIVE@#To search the objective morphologic evidence for the forensic pathological diagnosis on the acute myocardial ischemia/reperfusion.@*METHODS@#The expression of Bcl-2 protein in the early stage of acute myocardial ischemia/reperfusion(I/R) in rats were studyed with immunohistochemistry.@*RESULTS@#There was no expression of Bcl-2 protein in control group. The expression of Bcl-2 protein began to increase in myocytes 0.5 h after I/R in the ischemic areas and the expression of Bcl-2 protein in inner layer was stronger than in outer layer. The expression of Bcl-2 protein appears firstly in the inner layer of myocardium and extended gradually toward the outer layer 1 h after I/R. It was found that the expression of Bcl-2 protein in outer layer was stronger than in inner layer, then the expression of Bcl-2 protein became weaken gradually.@*CONCLUSION@#Bcl-2 gene may be an important regulative gene in the process of apoptosis and the changes observed in this paper might be useful for the forensic pathological diagnosis on the early myocardial ischemia.