Investigate the relationship between postmortem interval (PMI) and the metabolic law of the amount of DNA in cells of rat / 法医学杂志

ABSTRACT

OBJECTIVE@#To investigate the relationship between postmortem interval (PMI) and the metabolic law of the amount of DNA in cells.@*METHODS@#After different PMI from the heart, liver, spleen and kidney were taken into pieces respectively, then centrifuged and digested to get suspending cells fluid. The amount of DNA of rats'viscera were detected by flow cytometry after stained by fluorescence, and also inspect the amount of DNA in different periods to find out the law of its variation.@*RESULTS@#It showed a descendent trend of the amount of DNA in cells after different PMI, especially in spleen.@*CONCLUSION@#The amount of DNA of all the viscera grows downwards after death, this might be applyed in forensic estimation of PMI.