A primary study on the gene expression profiling of human brain contusion by cDNA microarray / 法医学杂志
Journal of Forensic Medicine
;
(6): 77-80, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-983020
ABSTRACT
OBJECTIVE@#To screen the differential expression of oncogenes and tumors suppressed genes(OTS genes) after human brain contusion by cDNA microrarray.@*METHODS@#The total RNAs isolated from normal and contusion human brain tissues were purified by Oligotex to obtain mRNAs. Both sources of mRNAs were reversely transcribed to cDNAs with the incorporation of fluorescent dUTP to prepare the hybridization probes. The probe from normal tissue and the contusion brain tissue were labeled with Cy3-dUTP and Cy5-dUTP respectively. The mixed probes were hybridized to the BioDoor Chip OTS-2.2S, a cDNA microarray which contains 227 oncogenes and tumors suppressed genes. After high-stringent washing, the cDNA microarray was scanned for the fluorescent signals and showed differences between two tissues.@*RESULTS@#Among the 227 target genes, 3 genes including Human carcinoma associated HOJ-1 (HoJ-1), Human KIAAOO65 gene,Human retinoblastoma related protein (p107) gene, showed distinct deference in expression level between the human brain contusion tissue and normal tissue.@*CONCLUSION@#The 3 genes in the brain contusion was significantly the differential expression by OTS 2.2S cDNA microarray. Further analysis of these genes will be helpful to understand the molecular mechanism of brain injury and utilization in forensic medicine.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Lesões Encefálicas
/
Proto-Oncogenes
/
RNA Mensageiro
/
Marcadores Genéticos
/
Proteínas Proto-Oncogênicas
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Perfilação da Expressão Gênica
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Proteína p107 Retinoblastoma-Like
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2004
Tipo de documento:
Artigo
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