A preliminary study on estimation of postmortem interval according to beta-actin mRNA stability in rat / 法医学杂志
Journal of Forensic Medicine
;
(6): 19-20, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-983064
ABSTRACT
OBJECTIVE@#In order to find a new parameter to estimate the postmortem interval, beta-actin mRNA in lung and thoracic muscle of rats was detected at different time point postmortem.@*METHODS@#Rats were killed by neck dislocation and left in a temperature controlling system at 21 degrees C for 12 days postmortem. Total RNA in lung and thoracic muscle at different time point was extracted and beta-actin mRNA expression was examined by RT-PCR. Semi-quantification analysis of the image of electrophoresis was performed to confirm the changes of beta-actin mRNA expression.@*RESULTS@#beta-actin mRNA in lung of rats still could be detected at 12 days postmortem, but-disappeared in thoracic muscle at 8 days postmortem.@*CONCLUSION@#The expression of beta-actin mRNA in lung and thoracic muscle could be a new parameter for estimation of PMI.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Músculos Peitorais
/
Mudanças Depois da Morte
/
Fatores de Tempo
/
RNA Mensageiro
/
Actinas
/
Ratos Sprague-Dawley
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Medicina Legal
/
Pulmão
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2005
Tipo de documento:
Artigo
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