Concentration and determination of strychnine alkaloid in biological fluids / 法医学杂志
Journal of Forensic Medicine
;
(6): 36-38, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-983070
ABSTRACT
OBJECTIVE@#To establish a new method for determination of strychnine alkaloid in biological fluids based on molecularly imprinted polymers.@*METHODS@#A strychnine molecularly imprinted monolithic column was prepared by in-situ molecularly imprinted technique. The polymer was filled to a 1cm column, and a method was developed to concentrate and determine strychnine alkaloids in biological fluids.@*RESULTS@#the limit of detection of the method was 4.9 ng, and the recoveries were more than 92%. The relative standard deviations were smaller than 6.59%. The linear correlation coefficients of standard curves were 0.999 1 and 0.9966 respectively. This method was applied to concentrate and determine strychnine in plasma and urine of poisoned rabbit.@*CONCLUSION@#The new method could concentrate and simultaneously determine strychnine alkaloids in biological fluids, and it was applied to forensic toxicological analysis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Polímeros
/
Estricnina
/
Cromatografia Líquida de Alta Pressão
/
Sensibilidade e Especificidade
/
Alcaloides
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2005
Tipo de documento:
Artigo
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