Forensic applications of the sequencing of mitochondrial DNA cytochrome oxidase subunit I gene for sarcosaphagous flies (Diptera) in Huhhot and Dunhuang district / 法医学杂志
Journal of Forensic Medicine
; (6): 100-106, 2005.
Article
em Zh
| WPRIM
| ID: wpr-983081
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To solve the problems of identification of Sarcosaphagous flies and their larvae, pupas and eggs.@*METHODS@#Sarcosaphagous Flies (Diptera) Samples were collected on the corpses of rabbits in the Huhhot district and a pig in the Dunhuang district. A 278bp region in the cytochrome oxidase subunit I (CO I) gene in mtDNA was analysed by DNA sequencing, A neighbour-joining tree using the Tamura and Nei model of nucleotide substitution was also constructed using the MEGA2.1 package.@*RESULTS@#A 278 base pairs region of the gene for CO I encoding region of mtDNA of above all samples was showed less than 1% sequence divergence within species and about 3% divergence between species.@*CONCLUSION@#It is an effective, easy and accurate method to be used for identification of these Sarcosaphagous Flies (Diptera) to species group by sequencing the 278 base pairs region of the CO I encoding gene of mtDNA.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Filogenia
/
Pupa
/
Especificidade da Espécie
/
Suínos
/
DNA Mitocondrial
/
Sequência de Bases
/
Reação em Cadeia da Polimerase
/
Análise de Sequência de DNA
/
Complexo IV da Cadeia de Transporte de Elétrons
/
Dípteros
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Ano de publicação:
2005
Tipo de documento:
Article