A study on the relationship between the degradation of protein and the postmortem interval / 法医学杂志
Journal of Forensic Medicine
;
(6): 110-112, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-983084
ABSTRACT
OBJECTIVE@#To observe the degradation of actin and tubulin in the liver tissue of rats after death and to find an objective indicator of the postmortem interval (PMI).@*METHODS@#Female rats were killed under anesthesia by ether and incubated at 21 degrees C in a temperature controlled system to simulate postmortem changes for 18 days postmortem. Protein in the hepatic tissue was extracted, actin and tubulin were then examined by western blot. Thereafter, the semi-quantitative analysis of the image of western blot was performed.@*RESULTS@#Actin in the liver tissue of rats could be detected at 8 days postmortem, but could not be examined after 10 days postmortem. beta-tubulin rather than alpha-tubulin could be examined after 2 days postmortem, and beta-tubulin could not be examined at 4 days postmortem.@*CONCLUSION@#There is some difference in the degradation between actin and tubulin, their different preservation period postmortem may be used as a parameter for PMI estimation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Mudanças Depois da Morte
/
Fatores de Tempo
/
Tubulina (Proteína)
/
Western Blotting
/
Actinas
/
Ratos Sprague-Dawley
/
Medicina Legal
/
Fígado
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2005
Tipo de documento:
Artigo
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