Analysis of 37 drugs in whole blood by HPLC after solid phase extraction / 法医学杂志
Journal of Forensic Medicine
; (6): 349-352, 2006.
Article
em Zh
| WPRIM
| ID: wpr-983221
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To develop a specific, sensitive, reproducible SPE-HPLC method for the determination of 37 drugs in whole blood.@*METHODS@#With the doxapram as internal standard, Oasis column was used to extract drugs from whole blood. Two kinds of mobile phases were used in this study. Separations were achieved by a LiChrospher 100 RP-C18 (250 mm x 4.0 mm x 5 microm) column kept at 50 degrees C, the DAD detector was set at 230 nm and 250 nm.@*RESULTS@#The limit of detection were 1-30 ng/mL. The method showed excellent linearity and the linear correlation coefficient was > or =0.997 98. The relative standard deviation for between-day and within-day assay were <10%.@*CONCLUSION@#The method is effective, simple, reliable and has been used in real cases.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Papaverina
/
Procaína
/
Solventes
/
Prazosina
/
Preparações Farmacêuticas
/
Reprodutibilidade dos Testes
/
Cromatografia Líquida de Alta Pressão
/
Sensibilidade e Especificidade
/
Doxapram
/
Doxepina
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Ano de publicação:
2006
Tipo de documento:
Article