Study of Papaver somniferum cultivars identification by TD-RAPD technique / 法医学杂志

ABSTRACT

OBJECTIVE@#The feasibility of Papaver somniferum L. cultivars identification was explored by TD-RAPD technique.@*METHOD@#Genomic DNA was extracted by improved CTAB method. One sample of species from Papaver somniferum L in xishuangbanna area. was studied by using TD-RAPD method.@*RESULT@#We established an optimal method of extracting genomic DNA. Six primers were picked out from 10 primers.@*CONCLUSION@#TD-RAPD could be applied to researches of molecular marker of Papaver somniferum L. TD-RAPD technique provide a method to constitute DNA database of Papaver somniferum L. and conclude the source of opium poppy.