Sequence-length polymorphism research of C-stretch in mitochondrial DNA hyper-variant region / 法医学杂志
Journal of Forensic Medicine
;
(6): 123-126, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-983281
ABSTRACT
OBJECTIVE@#To investigate the polymorphism of C-stretch in mitochondrial DNA hyper-variant region and the values in forensic science.@*METHODS@#The gene frequency were detected in such two loci of nt16180 and nt310 by using PCR-sequencing technique with two pair of primers according to the reference.@*RESULTS@#The length of PCR products were 807 bp and 962 bp respectively. Seven genotypes in each locus were detected. AAAACCCCCTCCCC allele amounted to 87.72% of all nt16180 genotype, while AAAACCCCCCCCCCCCC was firstly reported in Han population. CCCCCCCCTCCCCCC allele amounted to 60.53% of all nt310 genotype. 15 haplotypes were detected among 114 samples. The GD value was 0.6309. The AAAACCCCCTCCCC-CCCCCCCCTCCCCCC haplotype was the most high frequency and amounted to 57.89%.@*CONCLUSION@#The obtained basic genetic data in two loci of nt16180 and nt310 of mtDNA will be useful for forensic science.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Polimorfismo Genético
/
Haplótipos
/
DNA Mitocondrial
/
China
/
Reação em Cadeia da Polimerase
/
Análise de Sequência de DNA
/
Primers do DNA
/
Alelos
/
Frequência do Gene
/
Genética Populacional
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2007
Tipo de documento:
Artigo
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