Application of immunofluorescence and sandwich ELISA with double-antibodies in detection of human rabies / 法医学杂志
Journal of Forensic Medicine
;
(6): 411-413, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-983328
ABSTRACT
OBJECTIVE@#To explore the application of immunofluorescence and sandwich ELISA with double-antibodies in detection of human rabies.@*METHODS@#The cerebrum, cerebellum, brainstem, and hippocampus of four patients died of rabies identified by clinical diagnosis were collected and kept in freezer at -70 degrees C or in formaldehyde solution separately. The rat brain tissue infected by CVS strain of rabies virus was used as a positive control and the brain tissue of a patient died of acute pancreatitis was used as a negative control.@*RESULTS@#Rabies virus was detected in the tissues kept in freezer at -70 degrees C and the positive control but was not detected in the tissues kept in formaldehyde solution and the negative control.@*CONCLUSION@#Immunofluorescence and Sandwich ELISA with double-antibodies could be used in detection of human rabies. The samples should be kept in deep frozen temperature condition instead of in formaldehyde solution.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Raiva
/
Vírus da Raiva
/
Kit de Reagentes para Diagnóstico
/
Preservação de Tecido
/
Encéfalo
/
Ensaio de Imunoadsorção Enzimática
/
Imunofluorescência
/
Sensibilidade e Especificidade
/
Hipocampo
/
Anticorpos Antivirais
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2007
Tipo de documento:
Artigo
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