Analysis of appropriate amount of template DNA for sinofiler kit by real time quantitative PCR technique / 法医学杂志
Journal of Forensic Medicine
;
(6): 129-133, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-983368
ABSTRACT
OBJECTIVE@#To explore the appropriate amount of template DNA for Sinofiler Kit.@*METHODS@#The DNA samples with ideally genotyped results by Sinofiler Kit were detected by real-time quantitative PCR assay.@*RESULTS@#It was shown that 1.29-1.51 ng of template DNA in 12.5 microL reaction volume was optimal for STR genotyping with Sinofiler Kit.@*CONCLUSION@#Real time quantitative PCR is an accurate and necessary technique for detection of appropriate amount of template DNA for different kits.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Kit de Reagentes para Diagnóstico
/
Moldes Genéticos
/
DNA
/
Repetições de Microssatélites
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Medicina Legal
/
Cabelo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2008
Tipo de documento:
Artigo
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