Analysis of paraquat in blood and urine by sodium borohydride/nickel chloride chemical reduction-gas chromatography/thermionic specific detector / 法医学杂志
Journal of Forensic Medicine
; (6): 429-432, 2008.
Article
em Zh
| WPRIM
| ID: wpr-983422
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To establish a new method for the analysis of paraquat in blood and urine by sodium borohydride/nickel chloride chemical reduction-gas chromatography/thermionic specific detector.@*METHODS@#An initial procedure of precipitation was performed by adding hydrochloric solution with sodium chloride and a mixture of chloroform and ethanol. Then the analyte contained in supernatant was reduced by a reduction system of sodium borohydride and nickel chloride and extracted by acetic ether. Ethyl paraquat (EPQ) was used as internal standard. GC/TSD was used to identify and quantify the analyte.@*RESULTS@#The limits of detection (S/N=3) in blood and urine were 0.002 and 0.004 microg/mL, respectively. The linear ranges were 0.050-30.0 microg/mL. Correlation coefficients in blood and urine were 0.999 and 0.998, respectively. The recoveries exceeded 80% both in blood and urine.@*CONCLUSION@#This method is applicable for quantification of paraquat in biological fluids.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Oxirredução
/
Paraquat
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Boroidretos
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Cromatografia Gasosa
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Sensibilidade e Especificidade
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Toxicologia Forense
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Herbicidas
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Níquel
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Ano de publicação:
2008
Tipo de documento:
Article