Construction of standard allelic ladder of miniSTR loci by molecular cloning / 法医学杂志
Journal of Forensic Medicine
;
(6): 106-108, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-983453
ABSTRACT
OBJECTIVE@#To investigate the genetic polymorphism of five miniSTR loci (D9S2157, D9S1122, D10S1435, D12ATA63, D2S1776) in Hebei Han population and to construct standard allelic ladders.@*METHODS@#Polymorphism of the five miniSTR loci in 120 unrelated individuals was analyzed by fluorescence PCR and ABI 310 Genetic Analyzer. Molecular cloning technique was employed to construct standard allelic ladder of the 5 loci.@*RESULTS@#Of the five miniSTR loci, 8, 8, 7, 5 and 8 alleles were found, respectively. The polymorphism information component were 0.790, 0.720, 0.750, 0.630 and 0.850, respectively.@*CONCLUSION@#The five loci have relatively abundant polymorphic information and their standard allelic ladders constructed by molecular cloning technique are useful in forensic science.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Polimorfismo Genético
/
China
/
Reação em Cadeia da Polimerase
/
Clonagem Molecular
/
Sequências de Repetição em Tandem
/
Povo Asiático
/
Alelos
/
Genética Forense
/
Frequência do Gene
/
Genética Populacional
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2009
Tipo de documento:
Artigo
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