Changes of ICAM-1 mRNA expression in rats' skeletal muscle after contusion / 法医学杂志
Journal of Forensic Medicine
;
(6): 161-163, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-983463
ABSTRACT
OBJECTIVE@#To investigate the expression of intercellular adhesion molecular-1 (ICAM-1) mRNA in contused skeletal muscle of rats using fluorescence in situ hybridization (FISH) and its relationship with the contusion interval.@*METHODS@#To make the contusion models with rats skeletal muscle. The samples were taken to extract mRNA at 0.5 h, 1 h, 6 h, 12 h, 18 h, 24 h, 30 h and from the control group after contusion respectively. FISH was performed on frozen section samples and the sections were observed using LSCM.@*RESULTS@#The expression of ICAM-1 mRNA peaked at 6 h in skeletal muscle after contusion. Its level fell to 3.46 times the level of control group at 18 h and then increased again.@*CONCLUSION@#The time-order expression of ICAM-1 mRNA in 30 hours after contusion is potentially useful for estimation of early wound age.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fatores de Tempo
/
Cicatrização
/
RNA Mensageiro
/
Distribuição Aleatória
/
Ratos Sprague-Dawley
/
Hibridização in Situ Fluorescente
/
Músculo Esquelético
/
Molécula 1 de Adesão Intercelular
/
Contusões
/
Patologia Legal
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2009
Tipo de documento:
Artigo
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