The microglia activation characteristics of MA-induced neurotoxicity in the rats striatum / 法医学杂志
Journal of Forensic Medicine
;
(6): 321-325, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-983492
ABSTRACT
OBJECTIVE@#To investigate the activation characteristics of microglia (MG) in the rats striatum with MA-induced neurotoxicity.@*METHODS@#Male Wistar rats were divided randomly into control group (n=24) and experimental group (n=24). The rats of experimental group were injected intraperitoneally with MA (15 mg/kg x 8 injections, at 12 hours interval). The rats of control group were administrated with saline. The tissues of striatum of two rat groups were harvested at 0.5 d, 1 d, 2 d, 3 d, 4 d, 5 d, 6 d and 7 d post initial administrations of MA or saline. The structure changes were observed by transmission electron microscopy and CD-11b immunohistochemistry. The ratio of activated MG was calculated and statistically analyzed.@*RESULTS@#In the control group, the morphological characteristics of the MG showed that the cell bodies were small with slender processes, high electronic density nucleus, and fewer organelles known as the "fork-type". In contrast, the MG in the MA-induced neurotoxicity group displayed larger cell body, shorter cell processes or disappeared, lower electronic density nucleus and rich organelles, resembling "bush-like" or "amoeba-like". The ratio of activated MG in control group was below 0.15 at all timepoints, whereas in the experimental group, the ratio of activated MG increased significantly from day 1 to day 7 (P<0.001).@*CONCLUSION@#The continuous MA stimulation of the CNS results in prominent MG activation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Coloração e Rotulagem
/
Fatores de Tempo
/
Imuno-Histoquímica
/
Microscopia Eletrônica de Varredura
/
Distribuição Aleatória
/
Ratos Wistar
/
Microglia
/
Corpo Estriado
/
Metanfetamina
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2009
Tipo de documento:
Artigo
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