Determination of ethyl glucuronide in blood by GC-MS/MS / 法医学杂志
Journal of Forensic Medicine
; (6): 431-433, 2009.
Article
em Zh
| WPRIM
| ID: wpr-983519
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To develop a method for determining ethyl glucuronide(EtG) in human blood with gas chromatograph-tandem mass spectrometry (GC-MS/MS).@*METHODS@#Human blood protein was precipitated with acetonitrile. The supernatant was transferred and air flow dried after centrifugated. The residue was derived with N, O-bis (trimethylsilyl)trifluoroacetamide (BSTFA), and analyzed with GC-MS/MS.@*RESULTS@#The detection limit of EtG in blood was 0.05 microg/mL. Calibration curve covered a span from 0.1-10 microg/mL with a good linear relationship (r = 0.999 9). The method showed a excellent performance when was used to authentic blood sample analysis for EtG.@*CONCLUSION@#The method is suitable for blood EtG analysis.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Consumo de Bebidas Alcoólicas
/
Biomarcadores
/
Reprodutibilidade dos Testes
/
Sensibilidade e Especificidade
/
Alcoolismo
/
Toxicologia Forense
/
Glucuronatos
/
Cromatografia Gasosa-Espectrometria de Massas
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Ano de publicação:
2009
Tipo de documento:
Article