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DNA degradation in nucleolus of skeletal muscle, heart, liver, kidney and brain in mice after death / 法医学杂志
Journal of Forensic Medicine ; (6): 161-164, 2010.
Artigo em Chinês | WPRIM | ID: wpr-983556
ABSTRACT
OBJECTIVE@#To study the change of DNA degradation in nucleolus of mice organs and its relationship with the postmortem interval, and to investigate a new accurate method to estimate the postmortem interval.@*METHODS@#Eight parameters of cell nuclei were chosen, including the head DNA level, the tail DNA level, the head radius, the tail length, the tail moment, the Olive moment, the head area and the tail area. The changes of DNA degradation were analyzed in skeletal muscle, myocardium, liver, kidney and brain in mice at different intervals (0-72 h postmortem) by using single-cell gel electrophoresis and fluorescent microscope connected with auto-analysis-image system.@*RESULTS@#The tail DNA level, the tail length, the tail moment, the Olive moment and the tail area showed an increasing tendency. The head DNA level, the head radius and the head area showed a decreasing tendency within 72h postmortem in mice. A quadratic regression equation (P < 0.001) and multiple regression equation of DNA degradation tendency were established (P < 0.000 1).@*CONCLUSION@#The regression equations established can be used as a new method for estimating postmortem interval in forensic practice.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Mudanças Depois da Morte / Fatores de Tempo / Processamento de Imagem Assistida por Computador / DNA / Núcleo Celular / Músculo Esquelético / Ensaio Cometa / Miócitos Cardíacos / Patologia Legal / Rim Limite: Animais Idioma: Chinês Revista: Journal of Forensic Medicine Ano de publicação: 2010 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Mudanças Depois da Morte / Fatores de Tempo / Processamento de Imagem Assistida por Computador / DNA / Núcleo Celular / Músculo Esquelético / Ensaio Cometa / Miócitos Cardíacos / Patologia Legal / Rim Limite: Animais Idioma: Chinês Revista: Journal of Forensic Medicine Ano de publicação: 2010 Tipo de documento: Artigo