The suppression of c-fos in neuron apoptosis induced by heroin / 法医学杂志
Journal of Forensic Medicine
;
(6): 321-324, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-983586
ABSTRACT
OBJECTIVE@#To explore the changes of c-fos in apoptosis of cerebellar granular neuron of neonatal SD rats induced by heroin and the mechanisms of neuronal injury caused by heroin.@*METHODS@#Primary cerebellar granular neuron were cultured in vitro, the model of apoptosis induced by heroin was established. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were adopted to investigate the changes of c-fos in cell models.@*RESULTS@#Ten microg/mL of heroin was the optimal dose to induce the apoptosis of cerebellar granular neuron at 48 h. Both Western blotting and RT-PCR showed down regulation of c-fos expression.@*CONCLUSION@#Heroin could induce apoptosis of cerebellar granular neuron and down regulation of c-fos, which may be one of the apoptosis mechanisms.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA Mensageiro
/
Regulação para Baixo
/
Células Cultivadas
/
Cerebelo
/
Regulação da Expressão Gênica
/
Western Blotting
/
Proteínas Proto-Oncogênicas c-fos
/
Ratos Sprague-Dawley
/
Apoptose
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2010
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS